Laboratoř fotosyntézy
Skupina Ondřeje Prášila
Mikrobiální fotosyntéza
Mobility of photosynthetic proteins
We study mobility of photosynthetic proteins in thylakoid membrane of cyanobacteria and algae. Diffusion of proteins in thylakoid membrane is a key factor in several processes including state transitions and in de-novo protein synthesis. The protein movement is studied in situ on single cell level by confocal microscopy by means of FRAP (Fluorescence Recovery After Photobleaching). Out main aim is a characterization of interconnection between thylakoid membrane structure, protein dynamic and their function in photosynthesis. We focus mostly on a mobility of light harvesting antenna in algae and phycobilisomes in cyanobacteria or red algae.
The project is supported by GAČR P501-12-0304 (2012-2015)
RESULTS:
Kaňa R., Prášil O, Mullineaux CW (2009) Immobility of phycobilins in the thylakoid lumen of a cryptophyte suggests that protein diffusion in the lumen is very restricted. FEBS Lett. 583 (4), 670-674
Kaňa R., Prášil O., Komárek O., Papageorgiou G.C., Govindjee (2009) Spectral characteristic of fluorescence induction in a model cyanobacterium, Synechococcus sp. (PCC 7942). BBA – Bioenergetics, 1787 (10), 1170-1178.