Curriculum vitae

Mgr. Radek Kaňa, Ph.D.

ORCID: 0000-0001-5768-6902

Affiliation

Institute of Microbiology, Czech Academy of Sciences, Centre Algatech, Novohradská 237 - Opatovický mlýn, 379 81 Třeboň, CZ

Appointment

Scientist

Education and academic qualification

• 1995 – 2000, Palacký University, Olomouc, CZ;  Master studies of Biophysics - Photosynthesis
• 2000 – 2007, Palacký University, Olomouc, CZ; Ph.D. studies of Biophysics - Photosynthesis
• 2002 – 2004, Junior Research Associate, University of South Bohemia, Institute of Physical Biology, Nové Hrady, CZ
• 2005 – 2006, Experienced Researcher Stay, Biological Research Center, The Hungarian Academy of Sciences, Szeged, Hungary
• 2003 – 2007, Ph.D. student, Laboratory of Biophysics, Palacký University, Olomouc, Czech Republic

Overview of employment

• 2007 – Present, Lecturer, University of South Bohemia, České Budějovice, CZ; Bioenergetics of membranes
• 2007 – 2010, Junior Researcher, Institute of Microbiology, Czech Academy of Sciences, Centre Algatech, Třeboň, CZ
• 2010 – Present, Scientist - Group leader, Institute of Microbiology, Czech Academy of Sciences, Centre Algatech, Třeboň, CZ 

Stays abroad

• 2005, Prof. Jansson, Course on Proteomics - INTRO2, Umea University, Sweden – 2 weeks
• 2005, Prof. Dekker, Course on Laser Spectroscopy - INTRO2, Vrije Universiteit, Netherlands – 2 weeks
• 2006, Prof. Imre Vass, Biological Research Center, The Hungarian Academy of Sciences, Szeged, Hungary – 1 year
• 2006, Prof. Bruno Robert, Vibrational Spectroscopy - INTRO2 - Sacley, CEA, France – 2 weeks
• 2007 and 2008, Prof. CW. Mullineaux, FRAP measurements, Queen Mary University of London, UK – 1 month
• 2009, Prof. G. Garab - Confocal Microscopy, Biological Research Center, The Hungarian Academy of Sciences, Szeged, Hungary –  2 weeks
• 2013 and 2014, EMBL Heildeberg, Advanced Microscopic Methods – FRAP, FCS – 3 weeks
• 2011 and 2014, Prof A. Ruban, Mechanism of NPQ - Queen Mary University – 3 months
• 2013 and 2014, Prof D. Kirilovski, CEA Sacley France – Photoprotection in cyanobacteria – 3 weeks

Collabrators

• Prof A. Ruban – Queen Mary University of London, UK. “Membrane proteins regulation”
• Dr. Stefanno Santabarbara - University of Milan, Italy – “Biophysics of proteins”
• Dr. György Vámosi – University of Debrecen, Hungary – “Microscopic FCS method”
• Dr. Gabor Steinbach – Cellular Imaging Laboratory, Szeged, Hungary – “Confocal Microscopy”
• Prof. Tomáš Polívka – University of South Bohemia, CZ – “Photoprotection in proteins“
• Prof. Bruno Robert – CEA, Sacley, France – “New type of super-resolution microscopy”
• Prof. Joanna Kagul – University of Warsaw, Poland – “Photosynthesis in red algae“
• Dr. Jesus Salgado – University of Valencia, Spain – “AFM characterisation of protein oligomers”
• Dr. Bernard Lepetit – University Konstanz, Germany – “Microdomains membrane in diatoms ”

Selected research articles (recent 5 years)

1. V. Krynicka, P. Skotnicova, P.J. Jackson, S. Barnett, J.F. Yu, A. Wysocka, R. Kana, M.J. Dickman, P.J. Nixon, C.N. Hunter, J. Komenda, FtsH4 protease controls biogenesis of the PSII complex by dual regulation of high light-inducible proteins, Plant communications, 4 (2023). IF = 8.59
2. Crepin A, Belgio E, Šedivá B, Trsková EK, Kaňa R (2022) Size and Fluorescence Properties of Algal Photosynthetic Antenna Proteins Estimated by Microscopy. Int J Mol Sci 23: 778. IF= 5.924
3. Kaňa R, Steinbach G, Sobotka R, Vámosi G, Komenda J (2021) Fast Diffusion of the Unassembled PetC1-GFP Protein in the Cyanobacterial Thylakoid Membrane. Life 11 IF= 3.817
4. G. Pascual-Aznar, G. Konert, M. Beckova, E. Kotabova, Z. Gardian, J. Knoppova, L. Bucinska, R. Kaňa, R. Sobotka, J. Komenda, Psb35 Protein Stabilizes the CP47 Assembly Module and Associated High-Light Inducible Proteins during the Biogenesis of Photosystem II in the Cyanobacterium Synechocystis sp. PCC6803, Plant Cell Physiol., 62 (2021) 178-190. IF= 4.937
5. M. Canonico, G. Konert, A. Crepin, B. Šedivá, R. Kaňa, Gradual Response of Cyanobacterial Thylakoids to Acute High-Light Stress-Importance of Carotenoid Accumulation, Cells, 10 (2021) 1916.
6. Crepin A, Cunill-Semanat E, Kuthanová Trsková E, Belgio E, Kaňa R (2021) Antenna Protein Clustering In Vitro Unveiled by Fluorescence Correlation Spectroscopy. Int J Mol Sci 22: 2969 IF= 5.924
7. Canonico M, Konert G, Kaňa R (2020) Plasticity of Cyanobacterial Thylakoid Microdomains Under Variable Light Conditions. Frontiers in Plant Science 11 - IF= 5.753
8. Strašková A, Steinbach G, Konert G, Kotabová E, Komenda J, Tichý M, Kaňa R (2019) Pigment-protein complexes are organized into stable microdomains in cyanobacterial thylakoids. Biochimica et Biophysica Acta (BBA) - Bioenergetics 1860 - IF= 3.465
9. Konert G, Steinbach G, Canonico M, Kaňa R (2019) Protein arrangement factor: a new photosynthetic parameter characterizing the organization of thylakoid membrane proteins. Physiologia Plantarum 166: 264-277 – IF = 4.04
10. Kuthanova Trskova E, Belgio E, Yeates AM, Sobotka R, Ruban AV, Kaňa R (2018) Antenna proton sensitivity determines photosynthetic light harvesting strategy. Journal of Experimental Botany 69: 4483-4493 – IF= 6.992

Recent reviews

1. Santabarbara S, Casazza AP, Belgio E, Kaňa R, Prášil O (2020) Light harvesting by long-wavelength Chlorophyll forms (red forms) in algae. In Book , Larkum et al. eds, Photosynthesis in Algae: Biochemical and Physiological Mechanisms. Springer, pp 261-297 – Book chapter
2. Kaňa R, Govindjee (2016) Role of Ions in the Regulation of Light-Harvesting. Frontiers in Plant Science 7 IF=4.298

Invited lectures, talks

• 2014, Selected speaker at Gordon Photosynthetic Conference (West Dover, USA) - " Photoprotection in light-harvesting antenna of Chromera velia: role of carotenoid violaxanthin"
• 2015, Selected speaker - Molecualr Biology of Cyanobacteria (Texel, Nederland) - "Mobility of cytochrome b6f"
• 2016, Selected speaker - 17th International Congress on Photosynthesis Research, Session: Membrane ultrastructure and dynamics - “Presence of thylakoid membrane microdomains in cyanobacteria”

Teaching

University of South Bohemia, České Budějovice, CZ - Course on Bioenergetics, Course on Photosynthesis; supervising of Bachelor, Master and Ph.D. students 

Obtained funding

• 2020, Infrastructural project of the Czech Academy of Sciences. Adaptation of Zeiss LSM 880 to superesolution microscopy (purchasing of Airyscan detector, Budget 3.684 mil CZK)
• 2015 – 2020, Project ALGAMIC, Czech Ministry of Education, Infrastructural project OP VaVpI, (CZ 1.05/2.1.00/19.0392 - budget 21.422 mil. CZK), founding a new confocal laboratory - R.K. responsible for confocal microscopy implementation
• 2019 – 2021, Principal investigator: Role of thylakoid membrane microdomains in photosynthesis (Czech Grant Agency - 16-10088S, Budget: 6.000 mil. CZK)
• 2016 – 2018, Principal investigator: Mobility of photosynthetic proteins and photosynthesis (Czech Grant Agency - 16-10088S, Budget: 4.944 mil. CZK).
• 2012 – 2015, Principal investigator: Mobility of photosynthetic proteins (Czech Grant Agency - GAP501/12/0304, Budget: 5.912mil. CZK)